Baumann K, Bode F
Curr Probl Clin Biochem. 1977;8:36-43.
Proximal convoluted tubules were microperfused with an iodine 125 egg-white-lysozyme containing perfusion solution. The intraluminal lysozyme concentration decreased about 10% per mm tubular length over a wide range of intraluminal lysozyme concentrations tested indicating a constant fractional reabsorption over a wide concentration range. Lysozyme reabsorption at a high intraluminal lysozyme concentration of 10 mg/ml indicated that the tubular protein uptake by endocytosis is a saturable process. High intraluminal concentrations of basic amino acids inhibited the cellular uptake of lysozyme, while neutral amino acids had no effect.
用含碘125标记的蛋清溶菌酶的灌注液对近端曲管进行微灌注。在所测试的广泛管腔内溶菌酶浓度范围内，管腔内溶菌酶浓度每毫米管长下降约10%，这表明在较宽的浓度范围内存在恒定的分数重吸收。在管腔内溶菌酶浓度为10 mg/ml的高浓度下，溶菌酶的重吸收表明通过胞吞作用进行的肾小管蛋白质摄取是一个可饱和的过程。高浓度的碱性氨基酸抑制溶菌酶的细胞摄取，而中性氨基酸则没有影响。
