Grafe M R, Leonard C M
J Neuropathol Exp Neurol. 1980 Sep;39(5):555-74. doi: 10.1097/00005072-198009000-00005.
Degenerating axons in the human brain were successfully impregnated with reduced silver methods. The appearance of degenerating fibers did not differ markedly with survival times of three weeks and two, six, and twelve years following cerebral infarction or contusion of the brain. Impregnated fibers were found only along the appropriate corticofugal pathways. Electron microscopic examination of Vibratome-cut, silver-stained sections demonstrated silver deposition almost exclusively within axon fragments. Previous studies using anterograde degeneration methods in the human brain have limited their choice of cases to those with short periods of survival. Relaxing the restriction of short survival times extends the range of cases which can be used to study neural connections which may be unique to, or different in, the human brain.
采用还原银法成功浸染了人脑中正在退化的轴突。在脑梗或脑挫伤后存活三周以及两、六和十二年的情况下，退化纤维的外观并无明显差异。仅在合适的皮质传出通路上发现了浸染的纤维。对振动切片机切割的银染切片进行电子显微镜检查表明，银几乎完全沉积在轴突片段内。以往在人脑中使用顺行性变性方法的研究将病例选择局限于存活期短的病例。放宽对短存活时间的限制扩大了可用于研究可能为人脑所特有或与人脑不同的神经连接的病例范围。
