Kato N, Nakashima I, Ohta M, Nagase F, Yokochi T, Naito S
Microbiol Immunol. 1980;24(11):1043-51. doi: 10.1111/j.1348-0421.1980.tb02910.x.
The time course of development and decline of the ability of BCG-infected mice to produce interferon in the serum in response to the intravenous infection of purified protein derivative of tuberculin (PPD) was very similar to that of their systemic hypersensitivity to PPD. A cytotoxic factor (cytotoxin) was produced in parallel with interferon in the serum of BCG-infected mice after stimulation with PPD. The duration of the period in which cytotoxin-production responsiveness to PPD was definitely detectable was much shorter than that for interferon-production responsiveness although the periods for the maximum production of interferon and cytotoxin coincided. The kinetics of release of interferon in the serum of BCG-infected mice after stimulation with PPD did not parallel that of release of cytotoxin. The four kinds of activities, interferons and cytotoxins induced by PPD and lipopolysaccharide (LPS) in the serum of BCG-infected mice, were compared for their stability to heating at 56 C and to treatment at pH 2. The kinetics of inactivation of these four activities differed significantly, when the serum was either heated at 56 C or treated at pH 2. Interferon produced in response to LPS could be neutralized by anti-L cell(NDV) interferon rabbit serum as easily as L cell (NDV) interferon, 16 times as much antiserum was required to neutralize the same amount of interferon in response to PPD, but cytotoxins induced by PPD and LPS were not neutralized at all by the antiserum. From these findings it is thought likely that interferons and cytotoxins induced by PPD and LPS in the serum of BCG-infected mice are different substances, although the antigenic relationship between cytotoxins induced by PPD and LPS remains unknown.
卡介苗感染小鼠血清中产生干扰素以响应静脉注射结核菌素纯蛋白衍生物（PPD）的能力，其发展和衰退的时间进程与它们对PPD的全身超敏反应非常相似。在用PPD刺激后，卡介苗感染小鼠的血清中产生了一种细胞毒性因子（细胞毒素），其产生与干扰素平行。尽管干扰素和细胞毒素的最大产生期重合，但对PPD产生细胞毒素的反应性可明确检测到的持续时间比产生干扰素的反应性持续时间短得多。用PPD刺激后，卡介苗感染小鼠血清中干扰素的释放动力学与细胞毒素的释放动力学不平行。比较了卡介苗感染小鼠血清中由PPD和脂多糖（LPS）诱导的四种活性，即干扰素和细胞毒素，它们对56℃加热和pH 2处理的稳定性。当血清在56℃加热或在pH 2处理时，这四种活性的失活动力学有显著差异。对LPS产生的干扰素可以像L细胞（新城疫病毒）干扰素一样容易地被抗L细胞（新城疫病毒）干扰素兔血清中和，中和相同量的PPD诱导的干扰素需要16倍的抗血清，但PPD和LPS诱导的细胞毒素根本不能被抗血清中和。从这些发现可以认为，尽管PPD和LPS诱导的细胞毒素之间的抗原关系尚不清楚，但卡介苗感染小鼠血清中由PPD和LPS诱导的干扰素和细胞毒素是不同的物质。
