Lehmitz R, Meyer-Rienecker H J, Werner H, Eichler R
Psychiatr Neurol Med Psychol (Leipz). 1980 Oct;32(10):577-87.
The leukocyte migration inhibitory factor (LIF) was examined in 57 test persons--27 patients with multiple sclerosis (MS) and 30 normals--with the indirect and the direct leucocyte migration inhibition test in agarose (LMIAT). The stimulation of lymphocytes was carried out by the application of myelin basic protein (BP) and a membrane-associated antigen of normal brain (NTA). The mean value of controls +/- 2 s and the migration index less than or equal to 0,80 were used for limiting. In the indirect technique could be established significant group effects (NTA- and BP-values of MS cases compared to the controls) after 10 hours of incubation. The direct method showed in MS patients a significant lowered migration-index on an average after stimulation with the NTA; controls gave negative findings (except two cases). The valence of the test system and prospective modifications for further results were indicated.
采用琼脂糖白细胞迁移抑制试验（LMIAT）的间接法和直接法，对57名受试对象——27例多发性硬化症（MS）患者和30名正常人——进行了白细胞迁移抑制因子（LIF）检测。通过应用髓鞘碱性蛋白（BP）和正常脑组织的膜相关抗原（NTA）来刺激淋巴细胞。以对照组平均值±2s以及迁移指数小于或等于0.80作为界限。在间接技术中，孵育10小时后可确定显著的组间效应（将MS病例的NTA和BP值与对照组进行比较）。直接法显示，在用NTA刺激后，MS患者的迁移指数平均显著降低；对照组结果为阴性（除两例）。指出了该检测系统的效价以及为获得进一步结果可能进行的改进。
