Souliman S, Chapman I V
Int J Radiat Biol Relat Stud Phys Chem Med. 1981 Jan;39(1):31-8. doi: 10.1080/09553008114550041.
Hyperthermic treatment of HeLa cells at 42 degrees C for 60 min depressed the specific activity of these cells when incubated with 3H-uridine both during and post heating compared to cells maintained at 37 degrees C. These changes were unlikely to arise from increased leakage from the cells and may partially be attributed to membrane damage influencing facilitated diffusion. Diffusion kinetic data for incorporation of the radiolabel into the T.C.A. soluble and T.C.A. insoluble fractions of HeLa cells indicated that a significant depression of Vmax and a significant elevation of Km for incorporation of 3H UdR into RNA may possibly result from an isotope dilution effect attributed to degrading pre-ribosomal RNA under the effect of hyperthermia.
将HeLa细胞在42摄氏度下进行60分钟的热疗，与保持在37摄氏度的细胞相比，在加热期间和加热后与3H-尿苷一起孵育时，这些细胞的比活性降低。这些变化不太可能源于细胞渗漏增加，可能部分归因于影响易化扩散的膜损伤。将放射性标记掺入HeLa细胞的三氯乙酸可溶性和三氯乙酸不溶性部分的扩散动力学数据表明，3H UdR掺入RNA的Vmax显著降低和Km显著升高，这可能是由于在热疗作用下核糖体前体RNA降解导致的同位素稀释效应。
