Szewczuk M R, Campbell R J, Jung L K
J Immunol. 1981 Jun;126(6):2200-4.
The magnitude of the immune response in old and young mice to trinitrophenylated bovine gamma-globulin was measured in various lymphatic sites at a cellular level using the plaque-forming cell assay. As we have previously shown, the number of splenic IgM, IgG, and IgA anti-TNP PFC progressively declined in aging C57BL/6J male mice. In addition, mice receiving antigen in the 4 footpads and the base of the tail exhibited similar decline in the number of PFC in the draining peripheral lymph nodes with increasing age. In contrast, mesenteric and mediastinal lymph node IgM, IgG, and IgA anti-TNP PFC response to TNP-BGG in complete Freund's adjuvant, i.p. or via gastric intubation, in old mice remained unimpaired compared with the number in younger mice. The data support the view that the mucosal-associated lymphoid system differs from the systemic system with regard to immune competence with age. Furthermore, the findings imply a site preference for a decline in immune function with aging.
利用空斑形成细胞试验，在细胞水平上测定了老年和幼年小鼠对三硝基苯化牛γ球蛋白的免疫反应强度。正如我们之前所表明的，在衰老的C57BL/6J雄性小鼠中，脾脏中IgM、IgG和IgA抗TNP PFC的数量逐渐减少。此外，随着年龄的增长，在4个脚垫和尾巴基部接受抗原的小鼠，其引流外周淋巴结中PFC的数量也呈现出类似的下降趋势。相比之下，与幼年小鼠相比，老年小鼠经腹腔注射或通过胃插管给予完全弗氏佐剂中的TNP-BGG后，肠系膜和纵隔淋巴结中IgM、IgG和IgA抗TNP PFC的反应并未受到损害。这些数据支持了这样一种观点，即黏膜相关淋巴系统在免疫能力随年龄变化方面与全身系统不同。此外，这些发现意味着免疫功能随年龄下降存在部位偏好。
