Faure M, Eisinger M, Bystryn J C
J Invest Dermatol. 1981 May;76(5):347-51. doi: 10.1111/1523-1747.ep12520003.
The expression of upper cytoplasmic (U-CYT) antigens which are expressed only in the superficial layers of the epidermis and are markers of epidermal cell differentiation in vivo and of basement zone (BMZ) antigens reacting with bullous pemphigoid serum was studied in keratinocytes in tissue culture. The cells were cultured at an acid pH (5.6-5.8) similar to that of skin and without feeder cells, dermal tissue, or collagen. It was found that the expression of U-CYT antigens decreased markedly in culture. These antigens were expressed in 45-65% of epidermal cells prepared from fresh skin, but in only 5-10% of cells which had been grown in primary culture over 1 mo, and in no cells in secondary or tertiary culture. By contrast, BMZ antigens continued to be expressed in culture. These antigens were expressed by 20-35% of epidermal cells prepared from fresh tissue and by 15-35% of keratinocytes in primary, secondary or tertiary culture. These findings indicate that U-CYT and BMZ antigens can be used to type subpopulations of human keratinocytes in suspension, and suggest that the differentiation of these cells in vitro differs from that which occurs in vivo.
在组织培养的角质形成细胞中，研究了仅在表皮浅层表达的上细胞质（U-CYT）抗原的表达情况，这些抗原是体内表皮细胞分化的标志物，以及与大疱性类天疱疮血清反应的基底膜带（BMZ）抗原的表达情况。细胞在类似于皮肤的酸性pH值（5.6 - 5.8）下培养，且无饲养细胞、真皮组织或胶原蛋白。结果发现，U-CYT抗原的表达在培养过程中显著下降。这些抗原在从新鲜皮肤制备的表皮细胞中有45% - 65%表达，但在原代培养超过1个月的细胞中只有5% - 10%表达，在传代培养或三代培养的细胞中则无表达。相比之下，BMZ抗原在培养中持续表达。这些抗原在从新鲜组织制备的表皮细胞中有20% - 35%表达，在原代、传代或三代培养的角质形成细胞中有15% - 35%表达。这些发现表明，U-CYT和BMZ抗原可用于对悬浮状态的人角质形成细胞亚群进行分型，并提示这些细胞在体外的分化与体内发生的分化不同。
