Voronina A S, Bogatyreva S A
Mol Biol (Mosk). 1980;14(1):190-9.
New substances--lithium metatungstate (MTL) and tris-(hydroxymethyl)aminomethane phosphotungstate (PTT)--have been presented for density-gradient preparation. The buoyant densities of protein, RNA, DNA and some nucleoproteins were determined in solutions of these salts. Nucleic acids have been smaller buoyant density (1.1 g/cm3) than the proteins in contrast to CsCl-gradients. The protein in PTT solution have buoyant density 1.5 g/cm3 and in MTL solution 2.0-2,3 g/cm3. It was shown that MTL gradients allow to reach better resolution in nucleoprotein analysis than CsCl gradients.
新物质——偏钨酸锂（MTL）和三（羟甲基）氨基甲烷磷钨酸盐（PTT）已被用于密度梯度制备。在这些盐的溶液中测定了蛋白质、RNA、DNA和一些核蛋白的浮力密度。与CsCl梯度相反，核酸的浮力密度（1.1 g/cm³）比蛋白质小。PTT溶液中的蛋白质浮力密度为1.5 g/cm³，MTL溶液中的蛋白质浮力密度为2.0 - 2.3 g/cm³。结果表明，在核蛋白分析中，MTL梯度比CsCl梯度能达到更好的分辨率。
