Slaughter C A, Coseo M C, Cancro M P, Harris H
Proc Natl Acad Sci U S A. 1981 Feb;78(2):1124-8. doi: 10.1073/pnas.78.2.1124.
Six monoclonal antibodies against human placental alkaline phosphate [orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1], a highly polymorphic enzyme, were tested for reactivity against a panel of 295 placental extracts that had been typed electrophoretically. The products of the three common alleles as well as several rare alleles could be discriminated by the various antibodies. In some cases differences between allelic products were reflected by essentially "all-or-none" reactions, but in other cases the differences were smaller and demonstrable only by quantitative analysis of the binding. Evidence for allelic differences not detectable electrophoretically was also obtained.
针对人胎盘碱性磷酸酶[正磷酸单酯磷酸水解酶(最适碱性),EC 3.1.3.1](一种高度多态性的酶)的六种单克隆抗体,对一组经电泳分型的295份胎盘提取物进行了反应性测试。三种常见等位基因以及几种罕见等位基因的产物可通过不同抗体加以区分。在某些情况下,等位基因产物之间的差异通过基本的“全或无”反应得以体现,但在其他情况下,差异较小,只有通过结合的定量分析才能证明。还获得了电泳无法检测到的等位基因差异的证据。