Kennett R H
In Vitro. 1981 Dec;17(12):1036-50. doi: 10.1007/BF02618601.
Since the first report of hybridomas producing monoclonal antibodies by Kohler and Milstein in 1975, this technique has spread to nearly all areas of biological, biochemical, and biomedical research. Watching the use of these methods spread from immunologists to cell biologists, developmental biologists, biochemists and to other biological disciplines and observing the nearly logarithmic increase in publications using these reagents has been in itself fascinating and informative. An overview of the development of this technology and its applications is presented including the use of monoclonal antibodies to study cell surface molecules, differentiation antigens, receptors, and histocompatibility antigens. The use of these antibodies to analyze microorganisms and parasitic antigens as well as their use in the genetic analysis of human cell surface antigens and the detection of polymorphic variation in enzymes and other proteins is discussed. Examples of the application of monoclonal reagents to the study of tumor cell biology including the labeling of metastatic tumor cells and the detection of cell surface molecules implicated in the regulation of growth control and cell division are provided.
自1975年科勒和米尔斯坦首次报道杂交瘤产生单克隆抗体以来，这项技术已传播到生物学、生物化学和生物医学研究的几乎所有领域。目睹这些方法从免疫学家传播到细胞生物学家、发育生物学家、生物化学家以及其他生物学学科，并观察到使用这些试剂的出版物数量几乎呈对数增长，其本身就既引人入胜又信息丰富。本文概述了这项技术的发展及其应用，包括使用单克隆抗体研究细胞表面分子、分化抗原、受体和组织相容性抗原。讨论了利用这些抗体分析微生物和寄生虫抗原，以及它们在人类细胞表面抗原的遗传分析和酶及其他蛋白质多态性变异检测中的应用。还提供了单克隆试剂在肿瘤细胞生物学研究中的应用实例，包括转移性肿瘤细胞的标记以及与生长控制和细胞分裂调节相关的细胞表面分子的检测。
