Watson E L, Farnham C J, Friedman J, Farnham W
Am J Physiol. 1981 May;240(5):C189-92. doi: 10.1152/ajpcell.1981.240.5.C189.
Isolated mouse parotid acinar cells (acini) were prepared by enzyme digestion, divalent cation depletion, and mechanical shearing. Acini were found to be morphologically intact, i.e., 95% viable as judged by trypan blue exclusion. Amylase release by the cholinergic agonist carbachol, by the beta-adrenergic agonist isoproterenol, and by monensin was similar to responses obtained in mouse parotid fragments. Monensin-stimulated amylase release was associated with enhanced 22Na+ uptake and 45Ca2+ efflux; monensin did not affect 45Ca2+ uptake. In the absence of extracellular Na+, the response to monensin (50 microM) was reduced from 162 +/- 33.5 to 12.4 +/- 0.5%; monensin also failed to stimulate 45Ca2+ efflux. Similar results were obtained with isoproterenol (10(-6) M). The results suggest that Na+ ions may play a role in amylase release possibly by releasing Ca2+ from internal stores.
通过酶消化、二价阳离子去除和机械剪切制备分离的小鼠腮腺腺泡细胞（腺泡）。发现腺泡在形态上是完整的，即通过台盼蓝排斥法判断有95%的细胞存活。胆碱能激动剂卡巴胆碱、β-肾上腺素能激动剂异丙肾上腺素和莫能菌素诱导的淀粉酶释放与在小鼠腮腺片段中获得的反应相似。莫能菌素刺激的淀粉酶释放与增强的22Na+摄取和45Ca2+外流有关；莫能菌素不影响45Ca2+摄取。在没有细胞外Na+的情况下，对莫能菌素（50 microM）的反应从162±33.5%降低到12.4±0.5%；莫能菌素也未能刺激45Ca2+外流。用异丙肾上腺素（10(-6) M）也得到了类似的结果。结果表明，Na+离子可能通过从内部储存库释放Ca2+而在淀粉酶释放中发挥作用。
