福斯高林对小鼠腮腺胆碱能介导的淀粉酶释放的增强作用。

Augmentation of cholinergic-mediated amylase release by forskolin in mouse parotid gland.

作者信息

Watson E L, Singh J C, Jacobson K L

出版信息

Life Sci. 1985 Dec 30;37(26):2531-7. doi: 10.1016/0024-3205(85)90611-3.

Abstract

Cholinergic-mediated amylase release in mouse parotid acini was augmented by forskolin; the potency but not the maximal response to carbachol was altered. Amylase released by carbachol plus forskolin was dependent on extracellular calcium and was mimicked by the calcium ionophore, A23187 plus forskolin. Forskolin was also shown to enhance carbachol-stimulated 45Ca2+ uptake into isolated acini. Hydroxylamine, nitroprusside, and 8-bromo-c-GMP each in combination with forskolin mimicked the effects of carbachol plus forskolin on amylase release. In the presence of carbachol (10(-8)M) forskolin did not augment c-AMP levels. However, in the presence of carbachol (5 X 10(-7) M) or hydroxylamine (50 microM) forskolin did significantly augment c-AMP accumulation. These results suggest that calcium and c-GMP may mediate the augmentation of cholinergic-mediated amylase release by effects on c-AMP metabolism.

摘要

毛喉素增强了小鼠腮腺腺泡中胆碱能介导的淀粉酶释放；卡巴胆碱的效力改变，但最大反应未变。卡巴胆碱加毛喉素释放的淀粉酶依赖于细胞外钙，且可被钙离子载体A23187加毛喉素模拟。毛喉素还被证明可增强卡巴胆碱刺激的45Ca2+摄取到分离的腺泡中。羟胺、硝普钠和8-溴环鸟苷酸分别与毛喉素联合使用，模拟了卡巴胆碱加毛喉素对淀粉酶释放的作用。在存在卡巴胆碱（10(-8)M）的情况下，毛喉素不会增加环磷酸腺苷（c-AMP）水平。然而，在存在卡巴胆碱（5×10(-7)M）或羟胺（50μM）的情况下，毛喉素确实显著增加了c-AMP的积累。这些结果表明，钙和环鸟苷酸（c-GMP）可能通过对c-AMP代谢的影响来介导胆碱能介导的淀粉酶释放的增强。