Kumagai T
Endocrinol Jpn. 1980 Oct;27(5):551-60. doi: 10.1507/endocrj1954.27.551.
Immunohistochemical characterization of the human pituitary beta(R) cells was investigated through the findings of the immunoreactivities with anti-porcine ACTH, -rat TSH, -rat FSH sera. Immunostained corticotrophs are oval or round in shape and localized in the anteromedial wedge. It is shown on the adjacent sections that they correspond to the beta(R) cells with amphophilic stainability with PAS-iron hematoxylin. In this wedge, amphophilic cells are preponderant, but PAS-positive thyrotrophs and gonadotrophs are not numerous. Amphophilic stainability varies in degree from cell to cell: One cell contains numerous medium-size of secretory granules weakly stained with iron hematoxylin and strongly with PAS in the PAS-positive cytoplasm, and the other cell is filled with big secretory granules intensively stained with iron hematoxylin and weakly with PAS. The immunostained TSH, LH and FSH cells are different from the beta(R) corticotrophs, because anti-ACTH serum never reacts to the TSH, LH and FSH cells in the two adjacent sections. LH and FSH reactivities are observed in the single cells. It is concluded that human corticotrophs are amphophilic beta(R) cells filled with secretory granules, and that they have quite a different appearance from the rat chromophobic stellate corticotrophs with a row arrangement of secretory granules along the plasma membrane.
通过抗猪促肾上腺皮质激素、抗大鼠促甲状腺激素、抗大鼠促卵泡激素血清的免疫反应结果，对人垂体β（R）细胞进行了免疫组织化学特征研究。免疫染色的促肾上腺皮质激素细胞呈椭圆形或圆形，位于前内侧楔形区域。相邻切片显示，它们对应于用PAS-铁苏木精染色呈嗜双色性的β（R）细胞。在这个楔形区域中，嗜双色性细胞占优势，但PAS阳性的促甲状腺激素细胞和促性腺激素细胞数量不多。嗜双色性的程度在细胞之间有所不同：一个细胞含有许多中等大小的分泌颗粒，在用铁苏木精染色时颜色较浅，而在用PAS染色时，其阳性细胞质中颜色较深；另一个细胞则充满了大的分泌颗粒，在用铁苏木精染色时颜色较深，而在用PAS染色时颜色较浅。免疫染色的促甲状腺激素、促黄体生成素和促卵泡激素细胞与β（R）促肾上腺皮质激素细胞不同，因为在相邻的两个切片中，抗促肾上腺皮质激素血清从不与促甲状腺激素、促黄体生成素和促卵泡激素细胞发生反应。促黄体生成素和促卵泡激素的反应性在单个细胞中观察到。结论是人促肾上腺皮质激素细胞是充满分泌颗粒的嗜双色性β（R）细胞，并且它们与大鼠的无色素星状促肾上腺皮质激素细胞外观有很大不同，后者的分泌颗粒沿质膜呈一排排列。
