Umezu C, Yoshioka N, Takahashi K
Stain Technol. 1981 Mar;56(2):95-7. doi: 10.3109/10520298109067289.
Specific staining of sialic acid components after sodium dodecyl sulfate polyacrylamide gel electrophoresis can be carried out as follows: 1) extract glycoprotein of erythrocyte membranes or serum by the phenol-saline method, 2) electrophorese the extract on 5% polyacrylamide gel containing 0.1% sodium dodecyl sulfate at constant current, 3) treat the gel with chilled 0.04 M HIO4 for 45 minutes, 4) replace the periodic acid solution with one containing resorcinol 0.6 g, conc. HCl 50 ml, 0.1 M CuSO4 0.5 ml and H2O 50 ml, 5) warm the container in boiling water until blue violet sialic acid bands become clear, 6) replace the staining solution with a mixture of equal parts water and concentrated HCl and observe at once.
1）用酚-盐水法提取红细胞膜或血清中的糖蛋白；2）将提取物在含0.1%十二烷基硫酸钠的5%聚丙烯酰胺凝胶上恒流电泳；3）用冰冷的0.04M高碘酸处理凝胶45分钟；4）用含有0.6g间苯二酚、50ml浓盐酸、0.5ml 0.1M硫酸铜和50ml水的溶液替换高碘酸溶液；5）将容器在沸水中加热，直到蓝紫色的唾液酸条带清晰可见；6）用等份水和浓盐酸的混合液替换染色液并立即观察。
