Otsuki M, Sakamoto C, Ohki A, Yuu H, Morita S, Baba S
Am J Physiol. 1981 Jul;241(1):G43-8. doi: 10.1152/ajpgi.1981.241.1.G43.
The effects of both synthetic and pure natural porcine secretin on immunoreactive insulin (IRI) and immunoreactive glucagon (IRG) release and on pancreatic exocrine secretion were studied in the isolated perfused rat pancreas. Synthetic porcine secretin stimulated a significant increase in pancreatic juice flow and amylase output at concentrations as low as 0.01 and 0.1 ng/ml, respectively. The maximal peak rate of both juice flow and amylase output was observed at 1 microgram/ml synthetic secretin. Synthetic secretin at concentrations up to 2 micrograms/ml and pure natural porcine secretin at a concentration of 1 clinical unit/ml had no effect on IRI secretion regardless of the glucose concentration (50, 100, or 150 mg/100 ml) in the perfusate. Both types of secretin, however, elicited a concentration-dependent increase in IRG secretion in the presence of 50 mg/100 ml glucose. The lowest synthetic secretin concentration causing a significant increase in IRG release was 0.1 ng/ml, and maximal stimulation was observed at 1 microgram/ml. These concentrations were similar to those eliciting minimal and maximal amylase release. Thus, synthetic and pure natural porcine secretin have been shown to not only stimulate pancreatic juice flow and amylase secretion but also to elicit IRG release from the isolated perfused rat pancreas.
在离体灌注大鼠胰腺中，研究了合成猪促胰液素和纯天然猪促胰液素对免疫反应性胰岛素（IRI）和免疫反应性胰高血糖素（IRG）释放以及胰腺外分泌的影响。合成猪促胰液素分别在低至0.01和0.1 ng/ml的浓度下，可显著刺激胰液分泌量和淀粉酶分泌量增加。在1μg/ml合成促胰液素时，观察到胰液分泌量和淀粉酶分泌量的最大峰值速率。高达2μg/ml的合成促胰液素和1临床单位/ml的纯天然猪促胰液素，无论灌注液中葡萄糖浓度（50、100或150 mg/100 ml）如何，对IRI分泌均无影响。然而，在存在50 mg/100 ml葡萄糖的情况下，两种类型的促胰液素均引起IRG分泌呈浓度依赖性增加。引起IRG释放显著增加的最低合成促胰液素浓度为0.1 ng/ml，在1μg/ml时观察到最大刺激作用。这些浓度与引起最小和最大淀粉酶释放的浓度相似。因此，合成猪促胰液素和纯天然猪促胰液素不仅能刺激胰液分泌和淀粉酶分泌，还能引起离体灌注大鼠胰腺释放IRG。
