多发性硬化症脑提取物中的髓鞘碱性蛋白抗体。
Antibody to myelin basic protein in extracts of multiple sclerosis brain.
作者信息
Bernard C C, Randell V B, Horvath L B, Carnegie P R, Mackay I R
出版信息
Immunology. 1981 Jul;43(3):447-57.
Autoimmunity to a neural antigen is a suspected cause of multiple sclerosis (MS), and a candidate autoantigen is myelin basic protein (MBP). Accordingly, saline extracts of brain from patients with MS and other diseases were prepared and the content of immunoglobulin (Ig) determined. Antibody to MBP was measured with a highly-sensitive solid-phase radioimmunoassay using 125I-staphylococcal Protein A. Anti-MBP activity was detected in brain extracts of all eleven MS patients, and in seven out of the eight brain extracts from the patients with other diseases; however the level of anti-MBP activity was significantly higher in the MS extracts (P less than 0.01). Analysis of the MS brain extracts after purification by affinity chromatography columns revealed that the anti-MBP activity was specifically mediated by IgG and resided in the IgG1, IgG2, and/or IgG4 subclasses.
对神经抗原的自身免疫被怀疑是多发性硬化症（MS）的病因之一，髓鞘碱性蛋白（MBP）是一种候选自身抗原。因此，制备了MS患者和其他疾病患者大脑的盐水提取物，并测定了免疫球蛋白（Ig）的含量。使用125I-葡萄球菌蛋白A通过高灵敏度固相放射免疫测定法测量针对MBP的抗体。在所有11例MS患者的脑提取物中以及8例其他疾病患者的脑提取物中的7例中检测到了抗MBP活性；然而，MS提取物中的抗MBP活性水平显著更高（P小于0.01）。通过亲和色谱柱纯化后的MS脑提取物分析表明，抗MBP活性由IgG特异性介导，存在于IgG1、IgG2和/或IgG4亚类中。
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