Roux K H
J Immunol. 1981 Aug;127(2):626-32.
An antiserum recognizing previously undescribed antigenic determinants (designated subgroup w) of the heavy chain VH region was produced by immunizing a rabbit, homozygous for the VH haplotype a1x-y- with IgG from another a1x-y- rabbit, suppressed for a1. The antiserum gives a single fused precipitin band against sera from homozygous rabbits suppressed for the a1, a2, or a3 allotypes. By gel diffusion analysis, the determinant was found to be distinct from previously described a, x, and y allotypes, and was detectable on IgG, F(ab')2 gamma and the gamma-heavy chain. Radioinhibition analysis revealed 2 patterns of inhibition suggesting 2 variants of w that are closely linked to VH allotypes. Variant w34 was detected at very low levels (0.042 to 5.6 micrograms/mg IgG) in normal rabbits expressing the a1x-y-, a1-y33,30, and a3x32y- VH haplotypes. Variant w35 was detectable in a 2x32y33,- rabbits. Sera from homozygous a allotype-suppressed rabbits representing each of the 4 VH haplotypes similarly exhibited either w34 or w35 patterns of inhibition as described above. The level of w34 in a1- and a3-suppressed rabbits exhibited a dramatic 230-fold median increase. In contrast, w35 levels for a2-suppressed rabbits fell within the normal range. In addition to reacting with essentially all normal and suppressed sera, the antiserum reacted with preimmune serum from the same rabbit. Thus, anti-w represents an induced auto-antibody. These observations are further discussed with regard to rabbit and mouse VH populations.
通过用来自另一只a1x - y - 且a1被抑制的兔子的IgG免疫一只VH单倍型为a1x - y - 的纯合兔子，制备了一种抗血清，该抗血清可识别重链VH区域先前未描述的抗原决定簇（指定为w亚组）。该抗血清与来自a1、a2或a3同种异型被抑制的纯合兔子的血清产生一条单一融合沉淀带。通过凝胶扩散分析，发现该决定簇与先前描述的a、x和y同种异型不同，并且在IgG、F(ab')2γ和γ重链上均可检测到。放射性抑制分析揭示了两种抑制模式，表明w有两种变体，它们与VH同种异型紧密相连。在表达a1x - y - 、a1 - y33,30和a3x32y - VH单倍型的正常兔子中，以非常低的水平（0.042至5.6微克/毫克IgG）检测到变体w34。在2x32y33 - 兔子中可检测到变体w35。代表4种VH单倍型中每一种的纯合a同种异型被抑制兔子的血清同样表现出上述w34或w35抑制模式。a1和a3被抑制兔子中w34的水平中位数急剧增加了230倍。相比之下，a2被抑制兔子的w35水平在正常范围内。除了与基本上所有正常和被抑制的血清反应外，该抗血清还与同一只兔子的免疫前血清反应。因此，抗w代表一种诱导自身抗体。关于兔子和小鼠的VH群体，对这些观察结果进行了进一步讨论。
