Mikhaĭlov M V, Zasedatelev A S, Krylov A S, Gurskiĭ G V
Mol Biol (Mosk). 1981 May-Jun;15(3):690-705.
Experimental studies are reported on the binding of the dye "Hoechst 33 258" to DNA and synthetic polynucleotides. A stereochemical model of binding of the dye to DNA is proposed. According to the model, the molecules of the dye are located in the minor groove of DNA, each covering 4 base pairs. The benzimidazole backbone of the dye tends to form a helix isogeometric to that of DNA in the B form. The specifisity of "Hoechst 33 258" binding to At pairs involves hydrogen bonding of two NH-groups of benzimidazole rings and CH3N+H group of the piperazine ring to O2 oxygenes of thymines and N3 nitrogens of adenines facing the minor groove of the DNA double helix.
报道了关于染料“Hoeschst 33258”与DNA及合成多核苷酸结合的实验研究。提出了该染料与DNA结合的立体化学模型。根据该模型，染料分子位于DNA的小沟中，每个分子覆盖4个碱基对。染料的苯并咪唑主链倾向于形成与B型DNA螺旋几何形状相同的螺旋。“Hoeschst 33258”与A-T碱基对结合的特异性涉及苯并咪唑环的两个NH基团和哌嗪环的CH3N+H基团与面向DNA双螺旋小沟的胸腺嘧啶的O2氧和腺嘌呤的N3氮形成氢键。
