Kyegombe D B, Njoroge D K, Dossaji S F, Telang B V
Acta Biol Acad Sci Hung. 1977;28(4):443-50.
An acetylcholine-like substance was isolated from desiccated Jameson's mamba venom by one-dimensional ascending paper chromatography. The migratory and staining properties of the substance were identical with those of standard acetylcholine. Pharmacological identification on various in vitro and in vivo biological test objects showed that the substance was acetylcholine. It was further confirmed by high-voltage paper electrophoresis. The acetylcholine content was 4.35--4.36 mg/g of desiccated venom as shown by two different biological assay methods, and the index of discrimination was found to be 1.1, further confirming that substance in snake venom was acetylcholine.
通过单向上升纸色谱法从干燥的詹姆逊曼巴蛇毒中分离出一种类似乙酰胆碱的物质。该物质的迁移和染色特性与标准乙酰胆碱的特性相同。对各种体外和体内生物测试对象的药理学鉴定表明，该物质为乙酰胆碱。高压纸电泳进一步证实了这一点。两种不同的生物测定方法显示，干燥毒液中乙酰胆碱的含量为4.35 - 4.36毫克/克，鉴别指数为1.1，进一步证实蛇毒中的该物质为乙酰胆碱。
