Wurm F, Pauli G, Vielkind J
Cancer Res. 1981 Sep;41(9 Pt 1):3377-83.
In xiphophorine fish suffering from a genetically caused melanoma, both suppression of melanoma development and regression of the existing melanoma were observed after treatment of the fish with an "anti-melanoma immune RNA." This RNA was extracted from the lymphoid organs of guinea pigs immunized with fish melanoma. RNA from guinea pigs immunized with fish skin or liver and RNA from nonimmunized guinea pigs were ineffective. RNase treatment of the anti-melanoma immune RNA diminished its activity, although Pronase treatment did not. Analysis of antisera obtained from guinea pigs and rabbits immunized with either melanoma or normal skin of xiphophorine fish revealed differences in the immune responses induced by these tissues. The anti-melanoma sera recognized antigens in melanoma extracts, which were not present in skin extracts. These antigens were not recognized by the anti-skin sera. The results suggest specificity of the anti-melanoma immune RNA.
在用“抗黑色素瘤免疫RNA”处理患有遗传性黑色素瘤的剑尾鱼后，观察到黑色素瘤的发展受到抑制，现有的黑色素瘤也出现了消退。这种RNA是从用鱼黑色素瘤免疫的豚鼠的淋巴器官中提取的。用鱼皮或鱼肝免疫的豚鼠的RNA以及未免疫的豚鼠的RNA均无效果。用核糖核酸酶处理抗黑色素瘤免疫RNA会降低其活性，而用链霉蛋白酶处理则不会。对用剑尾鱼的黑色素瘤或正常皮肤免疫的豚鼠和兔子所获得的抗血清进行分析，发现这些组织诱导的免疫反应存在差异。抗黑色素瘤血清能识别黑色素瘤提取物中的抗原，而皮肤提取物中不存在这些抗原。这些抗原不能被抗皮肤血清识别。结果表明抗黑色素瘤免疫RNA具有特异性。
