Kregar I, Stanovnik B, Tisler M, Nisi C, Gubensek F, Turk V
Acta Biol Med Ger. 1977;36(11-12):1927-30.
Cathepsin D was inactivated with various diazo compounds at very high concentration. Reaction proceeded maximally at pH 4.5 in the presence of cupric ions. With 3-diazo-indazole and triazene the inactivation was noted also in the absence of cupric ions what indicates that the mechanism is mediated through triazene. CD-spectrum of partially inactivated enzyme shows that conformational changes occurred after treatment with diazo compound.
组织蛋白酶D在非常高的浓度下被各种重氮化合物灭活。在铜离子存在的情况下，反应在pH 4.5时进行得最为充分。对于3-重氮吲唑和三氮烯，在没有铜离子的情况下也观察到了灭活现象，这表明其作用机制是通过三氮烯介导的。部分灭活酶的圆二色光谱表明，在用重氮化合物处理后发生了构象变化。
