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古细菌核糖体蛋白的免疫学比较

Immunological comparison of ribosomal proteins from archaebacteria.

作者信息

Schmid G, Böck A

出版信息

J Bacteriol. 1981 Aug;147(2):282-8. doi: 10.1128/jb.147.2.282-288.1981.

DOI:10.1128/jb.147.2.282-288.1981
PMID:6167563
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC216043/
Abstract

Antisera were raised in rabbits against ribosomal proteins of Methanobacterium bryantii and used to analyze immunological relationships to ribosomes from other archaebacteria, from eubacteria, and from yeasts. Cross-reaction could be detected within the methanogens and with a member of the extreme halophiles; the degree of immunological similarity reflected the relationship delineated by 16S ribosomal ribonucleic acid oligonucleotide analysis (Fox et al., Science 209:457-463, 1980). With the methods and the anti-total-protein sera employed, there was no detectable cross-reaction with ribosomal proteins or ribosomes from Sulfolobus sp., eubacteria, or yeast.

摘要

在兔体内制备了抗布氏甲烷杆菌核糖体蛋白的抗血清，并用于分析其与其他古细菌、真细菌和酵母核糖体的免疫关系。在产甲烷菌内部以及与一种极端嗜盐菌之间可检测到交叉反应；免疫相似程度反映了由16S核糖体核糖核酸寡核苷酸分析所描绘的关系（福克斯等人，《科学》209:457 - 463，1980年）。采用所使用的方法和抗总蛋白血清，未检测到与来自硫化叶菌属、真细菌或酵母的核糖体蛋白或核糖体有交叉反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5205/216043/93880df3e44f/jbacter00267-0021-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5205/216043/0a655beb9b15/jbacter00267-0018-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5205/216043/b7b379a53511/jbacter00267-0020-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5205/216043/93880df3e44f/jbacter00267-0021-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5205/216043/0a655beb9b15/jbacter00267-0018-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5205/216043/b7b379a53511/jbacter00267-0020-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5205/216043/93880df3e44f/jbacter00267-0021-a.jpg

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引用本文的文献

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Electrophoretical and immunological comparison of the ribosomal proteins of the fungus Podospora anserina and the yeast Saccharomyces cerevisiae.真菌伞菌属和酵母酿酒酵母核糖体蛋白的电泳和免疫比较。
Curr Genet. 1984 Dec;9(1):53-8. doi: 10.1007/BF00396204.
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Selenocysteine, pyrrolysine, and the unique energy metabolism of methanogenic archaea.硒代半胱氨酸、吡咯赖氨酸与产甲烷古菌独特的能量代谢。
Archaea. 2010 Aug 17;2010:453642. doi: 10.1155/2010/453642.
3
Methanogens and the diversity of archaebacteria.产甲烷菌与古细菌的多样性

本文引用的文献

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Sequence homologies in the N-terminal region of the ribosomal 'A' proteins from Methanobacterium Thermoautotrophicum and Halobacterium cutirubrum.嗜热自养甲烷杆菌和红皮盐杆菌核糖体“A”蛋白N端区域的序列同源性。
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