Boyd J N, Stoewsand G S, Misslbeck N, Campbell T C, Mason R, Lepp C A, Odstrchel G
J Toxicol Environ Health. 1981 Jun;7(6):1025-35. doi: 10.1080/15287398109530042.
Male Fischer rats were fed semipurified diets containing 0, 1, 100, and 1000 ppm benzo[a]pyrene (BaP) for 6 or 13 wk. Plasma samples were assayed for alpha-fetoprotein (AFP) by a new sandwich-type radioimmunoassay (RIA) utilizing a special controlled porous-glass solid phase. This procedure is described in detail. Significant AFP elevation (p less than or equal to 0.01) was observed in the highest BaP treatment group after 5 wk of treatment. The 1 and 100 ppm Bap groups exhibited no AFP elevation throughout the study. Liver sections from the 1000 ppm groups had discrete gamma-glutamyl transpeptidase (GGT)-positive foci 10-20 cells in diameter by the 6th wk. GGT-positive foci were not evident in liver sections from the other treatment groups. Thus a high level of dietary BaP appears to rapidly alter rat liver cells, indicating hepatic neoplasia.
将雄性Fischer大鼠喂以含有0、1、100和1000 ppm苯并[a]芘（BaP）的半纯化日粮，持续6周或13周。通过一种新的夹心型放射免疫测定法（RIA），利用特殊的可控多孔玻璃固相，对血浆样本中的甲胎蛋白（AFP）进行测定。该方法有详细描述。在处理5周后，在最高BaP处理组中观察到显著的AFP升高（p≤0.01）。在整个研究过程中，1 ppm和100 ppm BaP组未出现AFP升高。到第6周时，来自1000 ppm组的肝脏切片有直径为10 - 20个细胞的离散γ-谷氨酰转肽酶（GGT）阳性病灶。在其他处理组的肝脏切片中未发现GGT阳性病灶。因此，高水平的日粮BaP似乎会迅速改变大鼠肝细胞，提示肝脏肿瘤形成。
