Slidders W, Robertson A J, Livesey A E, Cree I A, Brown R A, Beck J S
Microsc Acta. 1981 Jul;84(4):361-78.
In an attempt to quantitate histological structure by textural analysis, the characters of a stained microscopical field were recorded as a frequency polygon of the relative areas defined by successive increments of optical density: this is known as the optical density/area (OD/A) profile. In a study of lymph-nodes in Hodgkin's disease and of carcinoma of the female breast, each stained tissue section was found to have an OD/A profile of characteristic shape: moreover, in each of the lesions studied it was shown that the shape of the profile could be replicated by summing the profiles of each constituent cell type and intercellular matrix in proportions determined by differential cell counts and direct measurement of the degree of cell packing in the tissue. In an investigation of the influence of specimen preparation on OD/A profile, it was shown that density of staining and section thickness were important variables. Using the density of staining of tissue lymphocytes as an internal standard, a simple method was developed of compensating for differences in the density of Mayer's haemalum staining greater than those met with in practice. Attempts to compensate for intentionally large differences in section thickness were much less successful, but this was considered to be relatively unimportant since, in practice, the variation in thickness of sections cut on a modern microtome by a competent microtomist had been shown to be slight and to have a negligible effect on the OD/A profile.
为了通过纹理分析对组织结构进行定量，将染色显微镜视野的特征记录为光密度连续增量所定义的相对面积的频率多边形：这被称为光密度/面积（OD/A）曲线。在一项对霍奇金病淋巴结和女性乳腺癌的研究中，发现每个染色组织切片都有特征形状的OD/A曲线：此外，在所研究的每个病变中都表明，通过将每种组成细胞类型和细胞间基质的曲线按差异细胞计数和组织中细胞堆积程度的直接测量所确定的比例相加，可以复制该曲线的形状。在一项关于标本制备对OD/A曲线影响的研究中，表明染色密度和切片厚度是重要变量。以组织淋巴细胞的染色密度作为内标，开发了一种简单的方法来补偿迈尔苏木精染色密度大于实际遇到的差异。试图补偿故意设置的切片厚度的巨大差异则不太成功，但这被认为相对不重要，因为在实际操作中，熟练的切片技师在现代切片机上切出的切片厚度变化已被证明很小，对OD/A曲线的影响可以忽略不计。
