Jagodzinski L L, Sargent T D, Yang M, Glackin C, Bonner J
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3521-5. doi: 10.1073/pnas.78.6.3521.
We have determined the sequences of the recombinant DNA inserts of three bacterial plasmid cDNA clones containing most of the rat alpha a-fetoprotein mRNA. The resultant nucleotide sequence of alpha-fetoprotein was exhaustively compared to the nucleotide sequence of the mRNA encoding rat serum albumin. These two mRNAs have extensive homology (50%) throughout and the same intron locations. The amino acid sequence of rat alpha-fetoprotein has been deduced from the nucleotide sequence, and its comparison to rat serum albumin's amino acid sequence reveals a 34% homology. The regularly spaced positions of the cysteines found in serum albumin are conserved in rat alpha-fetoprotein, indicating that these two proteins may have a similar secondary folding structure. These homologies indicate that alpha-fetoprotein and serum albumin were derived by duplication of a common ancestral gene and constitute a gene family.
我们已经确定了三个细菌质粒cDNA克隆的重组DNA插入片段的序列，这些克隆包含了大鼠α-甲胎蛋白mRNA的大部分。由此得到的甲胎蛋白核苷酸序列与编码大鼠血清白蛋白的mRNA的核苷酸序列进行了详尽的比较。这两种mRNA在整体上具有广泛的同源性（50%），并且内含子位置相同。大鼠甲胎蛋白的氨基酸序列已从核苷酸序列推导得出，将其与大鼠血清白蛋白的氨基酸序列进行比较，发现同源性为34%。血清白蛋白中发现的半胱氨酸的规则间隔位置在大鼠甲胎蛋白中得以保留，这表明这两种蛋白质可能具有相似的二级折叠结构。这些同源性表明，甲胎蛋白和血清白蛋白是由一个共同的祖先基因复制而来，构成了一个基因家族。
