氯化甲基汞对完整大鼠和肾上腺切除大鼠肝脏中蛋白质和RNA合成的刺激作用。

Stimulation of protein and RNA synthesis by methylmercury chloride in the liver of intact and adrenalectomized rats.

作者信息

Omata S, Tsubaki H, Sakimura K, Sato M, Yoshimura R, Hirakawa E, Sugano H

出版信息

Arch Toxicol. 1981 Apr;47(2):113-23. doi: 10.1007/BF00332353.

Abstract

(1) A single injection of methylmercury chloride in the rat (10-50 mg/kg) increased both in vivo and in vitro rates of 14C-leucine incorporation into the protein of the post-mitochondrial supernatant fraction of the liver. In contrast, no stimulation of protein synthesis was observed in the brain of the methylmercury-treated rats. (2) Methylmercury administration also stimulated RNA polymerase activities in isolated hepatic nuclei, stimulation of Mg-dependent activity being higher than that of Mn-dependent activity. (3) In experiments with adrenalectomized rats, it was found that the stimulatory effect of methylmercury on protein and RNA synthesis in the liver was mediated partly through the adrenal gland. (4) Analysis of serum by starch-block electrophoresis revealed that synthesis of all serum proteins, including albumin and alpha-gamma globulin fractions, was stimulated by methylmercury treatment. (5) These results suggest that the observed effects of methylmercury on the liver depend on mechanisms other than enhancement of the synthesis of acute-phase proteins.

摘要

(1) 给大鼠单次注射氯化甲基汞（10 - 50毫克/千克）会提高肝脏线粒体后上清液组分蛋白质中14C-亮氨酸的体内外掺入率。相比之下，在经甲基汞处理的大鼠大脑中未观察到蛋白质合成的刺激作用。(2) 给予甲基汞还会刺激分离的肝细胞核中的RNA聚合酶活性，对镁依赖性活性的刺激高于对锰依赖性活性的刺激。(3) 在对肾上腺切除大鼠的实验中发现，甲基汞对肝脏蛋白质和RNA合成的刺激作用部分是通过肾上腺介导的。(4) 通过淀粉块电泳对血清进行分析表明，甲基汞处理会刺激包括白蛋白和α-γ球蛋白组分在内的所有血清蛋白的合成。(5) 这些结果表明，观察到的甲基汞对肝脏的影响取决于急性期蛋白合成增强以外的机制。