Githens S, Holmquist D R, Whelan J F, Ruby J R
Cancer. 1981 Mar 15;47(6 Suppl):1505-12. doi: 10.1002/1097-0142(19810315)47:6+<1505::aid-cncr2820471411>3.0.co;2-j.
Rat and hamster pancreatic ducts were isolated by digestion with collagenase plus chymotrypsin and were cultured for eight weeks in an agarose matrix. Freshly isolated and cultured ducts were characterized morphologically and biochemically. The in vivo morphology of the ducts was maintained in vitro, although certain differences were noted. Both interlobular and intralobular ducts could be identified. gamma-Glutamyltranspeptidase and Mg-ATPase were stable enzymatic activities of the ducts of both species; alkaline phosphatase persisted only in the hamster ducts. Carbonic anhydrase and (Na + K)ATPase were minor activities of the rat ducts. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the rat ducts suggested that actin was the major duct peptide and that the major zymogens were greatly diminished. These results demonstrate that pancreatic ducts can be maintained in vitro and can be used for biochemical studies of this minor pancreatic tissue type.
通过用胶原酶加胰凝乳蛋白酶消化分离大鼠和仓鼠的胰管，并在琼脂糖基质中培养八周。对新鲜分离和培养的胰管进行形态学和生物化学特征分析。尽管注意到了某些差异，但胰管的体内形态在体外得以维持。小叶间和小叶内胰管均可识别。γ-谷氨酰转肽酶和镁-ATP酶是两种动物胰管稳定的酶活性；碱性磷酸酶仅在仓鼠胰管中持续存在。碳酸酐酶和（钠+钾）ATP酶是大鼠胰管的次要活性。大鼠胰管的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳表明，肌动蛋白是主要的胰管肽，主要的酶原大大减少。这些结果表明，胰管可以在体外维持，并可用于对这种次要胰腺组织类型进行生化研究。
