Githens S, Finley J J, Patke C L, Schexnayder J A, Fallon K B, Ruby J R
Pancreas. 1987;2(4):427-38. doi: 10.1097/00006676-198707000-00010.
Pancreatic duct fragments were isolated from rat and hamster pancreas and were cultured in an agarose matrix for up to 8 weeks (rat) or 20 weeks (hamster). The fragments consisted predominantly of duct epithelium, lesser numbers of stromal and atrophied acinar cells, and small numbers of islet cells. Hamster ducts averaged 3 micrograms protein per duct while rat ducts averaged 1 microgram, and the protein:DNA ratio of both types of ducts was less than that of whole pancreas. Estimated average duct yields of 6% (hamster) and 1% (rat) were based on the protein content of the ducts. Duct viability was shown by the incorporation of 3H-thymidine and 3H-leucine into bulk DNA and protein and by autoradiography. gamma-Glutamyl transferase and (Na + K)-ATPase specific activities were slightly elevated while amylase was depressed in the ducts when compared with whole pancreas in both species. gamma-Glutamyl transferase was localized histochemically in both duct epithelium and in surviving acinar tissue, as seen in vivo. Amylase was shown by immunohistochemistry to be present within duct lumina and in atrophied acini and their lumina. Alkaline phosphatase and Mg-ATPase specific activities were elevated in the hamster, but reduced in the rat, when compared with whole pancreas. Hamster alkaline phosphatase and Mg-ATPase were localized by histochemistry to the duct stroma, where these enzymes are not detected in vivo. Carbonic anhydrase was found in the duct epithelium of both species, as in vivo, as well as in the duct stroma, unlike in vivo. Acid glycosaminoglycans, as revealed by alcian blue staining, were found at the apical surfaces and in the lumina of both kinds of ducts. Glutathione-S-transferase and glucose-6-phosphate dehydrogenase were elevated in rat ducts, but not in hamster ducts. The polypeptide compositions of cultured ducts, freshly isolated pancreatic islets, and whole pancreas were compared by one-dimensional sodium dodecyl sulfate polyacrylamide gradient gel electrophoresis. No duct-specific polypeptides were observed; the ducts were characterized mainly by the reduction or absence of polypeptides, including some zymogens, seen in whole pancreas.
从大鼠和仓鼠的胰腺中分离出胰管片段，并在琼脂糖基质中培养长达8周（大鼠）或20周（仓鼠）。这些片段主要由导管上皮组成，还有少量的基质细胞和萎缩的腺泡细胞以及少量的胰岛细胞。仓鼠胰管平均每条含3微克蛋白质，而大鼠胰管平均每条含1微克蛋白质，两种胰管的蛋白质与DNA比率均低于整个胰腺。根据胰管的蛋白质含量估算，仓鼠胰管的平均产量为6%，大鼠胰管为1%。通过将3H-胸腺嘧啶核苷和3H-亮氨酸掺入总DNA和蛋白质中以及放射自显影显示胰管的活力。与两种动物的整个胰腺相比，胰管中的γ-谷氨酰转移酶和（钠+钾）-ATP酶的比活性略有升高，而淀粉酶活性降低。如在体内所见，γ-谷氨酰转移酶通过组织化学定位在导管上皮和存活的腺泡组织中。免疫组织化学显示淀粉酶存在于导管管腔以及萎缩的腺泡及其管腔中。与整个胰腺相比，仓鼠的碱性磷酸酶和镁-ATP酶比活性升高，而大鼠的则降低。仓鼠的碱性磷酸酶和镁-ATP酶通过组织化学定位在导管基质中，而在体内未检测到这些酶。与体内不同，在两种动物的导管上皮以及导管基质中均发现了碳酸酐酶。通过阿尔辛蓝染色显示，酸性糖胺聚糖存在于两种导管的顶端表面和管腔中。大鼠胰管中的谷胱甘肽-S-转移酶和葡萄糖-6-磷酸脱氢酶升高，而仓鼠胰管中则未升高。通过一维十二烷基硫酸钠聚丙烯酰胺梯度凝胶电泳比较了培养的胰管、新鲜分离的胰岛和整个胰腺的多肽组成。未观察到胰管特异性多肽；胰管的特征主要是整个胰腺中所见的一些多肽（包括一些酶原）减少或缺失。
