Wehland J, Willingham M C, Dickson R, Pastan I
Cell. 1981 Jul;25(1):105-19. doi: 10.1016/0092-8674(81)90235-x.
Affinity-purified antibodies prepared against the major coat protein of brain coated vesicles, clathrin, were microinjected into cultured fibroblasts, and their intracellular distribution was followed by immunofluorescence microscopy and ultrastructural immunocytochemistry. Microinjected anticlathrin antibodies were concentrated on coated regions of the plasma membrane and the GERL apparatus. When an excess of anticlathrin antibodies was injected into the cytosol, coated pits on the plasma membrane were covered by anticlathrin antibody but still functioned to cluster an internalize alpha2-macroglobulin. These results are discussed in terms of the role of clathrin in the pathway of receptor-mediated endocytosis. Our data indicate that in cultured fibroblasts coated pits are stable elements permanently attached to the plasma membrane.
针对脑被膜小泡的主要外壳蛋白网格蛋白制备的亲和纯化抗体，被显微注射到培养的成纤维细胞中，通过免疫荧光显微镜和超微结构免疫细胞化学追踪其在细胞内的分布。显微注射的抗网格蛋白抗体集中在质膜和GERL装置的被膜区域。当过量的抗网格蛋白抗体被注射到细胞质中时，质膜上的被膜小窝被抗网格蛋白抗体覆盖，但仍能聚集并内化α2-巨球蛋白。根据网格蛋白在受体介导的内吞途径中的作用对这些结果进行了讨论。我们的数据表明，在培养的成纤维细胞中，被膜小窝是永久附着在质膜上的稳定结构。
