表皮生长因子通过高尔基体系统被膜小窝的转运。
Transit of epidermal growth factor through coated pits of the Golgi system.
作者信息
Willingham M C, Pastan I H
出版信息
J Cell Biol. 1982 Jul;94(1):207-12. doi: 10.1083/jcb.94.1.207.
Abstract
Using the direct conjugate of epidermal growth factor (EGF) and horseradish peroxidase, we have followed the entry of EGF into KB (human carcinoma) cells. EGF initially was found bound diffusely to the entire cell surface at 4 degrees C; on warming to 37 degrees C, EGF was found clustered in clathrin-coated pits on the plasma membrane in 1 min or less. Within 1-2 min at 37 degrees C, EGF began to accumulate in receptosomes within the cell and remained there for up to 10 min. At 10-13 min after warming to 37 degrees C, EGF was found in thin reticular membranous elements of the Golgi system, as well as concentrated in the clathrin-coated pits present on these membranes. By 15 min after warming, EGF began to be delivered to lysosomes located near the Golgi system. These findings suggest that clathrin-coated pits in the Golgi reticular system accumulate EGF before delivery to lysosomes.
摘要
利用表皮生长因子（EGF）与辣根过氧化物酶的直接共轭物，我们追踪了EGF进入KB（人癌细胞）细胞的过程。在4℃时，最初发现EGF广泛结合于整个细胞表面；升温至37℃后，在1分钟或更短时间内，发现EGF聚集在质膜上的网格蛋白包被小窝中。在37℃下1 - 2分钟内，EGF开始在细胞内的受体小体中积累，并在那里停留长达10分钟。升温至37℃后10 - 13分钟，发现EGF存在于高尔基体系统的薄网状膜结构中，并且集中在这些膜上的网格蛋白包被小窝中。升温后15分钟，EGF开始被递送至高尔基体系统附近的溶酶体。这些发现表明，高尔基体网状系统中的网格蛋白包被小窝在将EGF递送至溶酶体之前会积累EGF。
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