Seiler N, Sarhan S, Roth-Schechter B F
Dev Neurosci. 1981;4(3):181-7. doi: 10.1159/000112755.
Dissociated cells of cerebral hemispheres from 8-day-old chick embryos were cultivated for 8 days in polylysine-coated Petri dishes. Changes of DNA, RNA, total proteins, putrescine, spermidine, spermine, free amino acids and enzymes involved in polyamine and GABA metabolism were studied throughout neuronal development in culture. The presence of GABAergic neurons in the cultured cell population was demonstrated. There were time-dependent changes in cellular polyamine concentrations and the activities of the enzymes involved in polyamine metabolism. Since no significant proliferation took place after the first day in culture, the observed changes indicate a role of polyamine metabolism during neuronal differentiation; it was not possible, however, to attribute the observed changes to specific functions. This culture system seems especially useful for the study of biochemical and of functional correlations between GABA and polyamine metabolism and morphological and functional developments of neurons.
将8日龄鸡胚大脑半球的解离细胞在聚赖氨酸包被的培养皿中培养8天。在培养过程中，研究了神经元发育过程中DNA、RNA、总蛋白、腐胺、亚精胺、精胺、游离氨基酸以及参与多胺和GABA代谢的酶的变化。证实了培养细胞群体中存在GABA能神经元。细胞多胺浓度和参与多胺代谢的酶的活性存在时间依赖性变化。由于培养第一天后未发生明显增殖，观察到的变化表明多胺代谢在神经元分化过程中起作用；然而，不可能将观察到的变化归因于特定功能。这种培养系统似乎特别有助于研究GABA与多胺代谢之间的生化和功能相关性以及神经元的形态和功能发育。
