Sternlicht A L, Schultz R M
J Exp Zool. 1981 Feb;215(2):191-200. doi: 10.1002/jez.1402150209.
Kinetics of accumulation of total and poly(A)-containing RNA have been measured during growth of the mouse oocyte. Total RNA from oocytes isolated at discrete stages of growth was determined by two independent microassays. The full-grown oocyte contained about 0.60 ng of RNA. Kinetics of accumulation of total RNA with respect to oocyte volume were biphasic. Small, growing oocytes (about 30 pl) contained about 0.20 ng of RNA/oocyte. The amount of RNA increased in a quasi-linear fashion until oocyte volume was about 160 pl, at which point there was about 0.57 ng of RNA/oocyte. Thus oocytes about 65% of their final volume had accumulated about 95% of the total amount of RNA present in the fully-grown oocyte. The relative amount of poly (A)-containing RNA in oocytes of various size was determined by in situ hybridization of [3H] poly (U) to ovarian sections from juvenile mice of known age, followed by autoradiography. The kinetics of accumulation of poly (A)-containing RNA were similar to those of total RNA; oocytes about 70% of their final volume had accumulated about 95% of the amount of poly (A)-containing RNA present in the fully-grown oocyte. The poly(A)-containing RNA resided predominantly in the cytoplasm and no obvious cytoplasmic localization was observed. Kinetics of accumulation of total RNA, which is mainly ribosomal, and poly (A)-containing RNA were consistent with levels of RNA polymerases I and II measured by others during oocyte growth (Moore and Lintern-Moore, '78). The number of ribosomes that could be made from the amount of rRNA present at various stages of growth was compared to the actual number of ribosomes calculated from a published morphometric study (Garcia et al., '79). Kinetic differences in accumulation between the theoretical and actual number of ribosomes suggested oocyte ribosomes are recruited into cytoplasmic lattice structures. These structures accumulate during oocyte growth and have been postulated to be a ribosomal storage form. In addition, the results from this study are compared to results derived from lower species.
在小鼠卵母细胞生长过程中，已对总RNA和含聚腺苷酸（poly(A)）的RNA的积累动力学进行了测定。通过两种独立的微量测定法，确定了在不同生长阶段分离出的卵母细胞中的总RNA含量。完全成熟的卵母细胞含有约0.60 ng的RNA。总RNA相对于卵母细胞体积的积累动力学呈双相性。较小的正在生长的卵母细胞（约30 pl）每个卵母细胞含有约0.20 ng的RNA。RNA的量以准线性方式增加，直到卵母细胞体积约为160 pl，此时每个卵母细胞约有0.57 ng的RNA。因此，体积约为最终体积65%的卵母细胞已经积累了完全成熟卵母细胞中RNA总量的约95%。通过将[³H]聚尿苷（poly(U)）与已知年龄的幼年小鼠卵巢切片进行原位杂交，然后进行放射自显影，确定了不同大小卵母细胞中含poly(A)的RNA的相对量。含poly(A)的RNA的积累动力学与总RNA相似；体积约为最终体积70%的卵母细胞已经积累了完全成熟卵母细胞中含poly(A)的RNA量的约95%。含poly(A)的RNA主要存在于细胞质中，未观察到明显的细胞质定位。主要为核糖体RNA的总RNA和含poly(A)的RNA的积累动力学与其他人在卵母细胞生长过程中测得的RNA聚合酶I和II的水平一致（Moore和Lintern-Moore，1978年）。将在不同生长阶段存在的rRNA量可形成的核糖体数量与根据已发表的形态计量学研究计算出的实际核糖体数量进行了比较（Garcia等人，1979年）。理论核糖体数量与实际核糖体数量在积累上的动力学差异表明，卵母细胞核糖体被招募到细胞质晶格结构中。这些结构在卵母细胞生长过程中积累，并且被假定为一种核糖体储存形式。此外，将本研究的结果与来自低等物种的结果进行了比较。
