Fink D J, Russell J T, Gainer H, Brownstein M J, Baumgold J
J Neurobiol. 1981 Sep;12(5):487-503. doi: 10.1002/neu.480120508.
The transport of labeled proteins from the hypothalamus to the neurohypophysis following 35S-methionine injection into the rat supraoptic nucleus was studied using a unique approach adapted for the study of short-axon systems. Multiple-rate components to those found in other neuronal systems were demonstrated. Neurosecretory vesicle-containing proteins (e.g., neurophysins) were transported at fast rates (greater than 120 mm/day), whereas the cytoskeletal protein, actin, moved principally in the slow component of transport. Two-dimensional gel electrophoresis was used to analyze the diverse patterns of labeled proteins found in the various rate components of axonal transport in this system.
采用一种适用于研究短轴突系统的独特方法，研究了向大鼠视上核注射35S-蛋氨酸后标记蛋白从下丘脑向神经垂体的转运。结果显示，该系统中存在多种速率成分，与其他神经元系统中的情况不同。含神经分泌囊泡的蛋白质（如神经垂体素）以快速率（大于120毫米/天）转运，而细胞骨架蛋白肌动蛋白主要在转运的慢成分中移动。利用二维凝胶电泳分析了该系统轴突转运不同速率成分中发现的标记蛋白的多样模式。
