Aivazashvili V A, Bibilashvili R Sh, Vartikian R M, Kutateladze T A
Mol Biol (Mosk). 1981 Jul-Aug;15(4):915-29.
The elongation rate of RNAs synthesized from AI promoters of T7 phage DNA and its deletion mutant delta DIII T7 DNA by E. coli RNA polymerase was analyzed. The distribution of incorporation rates of any definite nucleotides at any definite position along the two RNA chains was studied. The minimal structure which reproducibly forms pauses seems to be trinucleotide. Two main groups of trinucleotides could be distinguished: 1) those mostly associated with pauses and; 2) those usually found in pause free regions. The first group consists of AUG, AUA, AUC, AAU, GUG, GUA, CGU, CGC, UUA, UUU; the second one comprises AAA, CAA, CCC, UCC, CUA, CUG, CUC, GGG, ACU, GAG, GAA, GGA. A model accounting for intermittent elongation has been developed. It is based on the hypothesis that the kinetic constants of each nucleotide incorporation to and pyrophosphorolysis from the 3'-end of the growing RNA chain depend on the nature of the incoming nucleotide as well as on the nature of a nucleotide residue situated at the 3'-end of the growing RNA. A general equation describing the pause distribution along the RNA of a known nucleotide sequence is proposed.
分析了大肠杆菌RNA聚合酶从T7噬菌体DNA的AI启动子及其缺失突变体δDIII T7 DNA合成的RNA的延伸率。研究了沿两条RNA链在任何确定位置的任何确定核苷酸的掺入率分布。可重复形成停顿的最小结构似乎是三核苷酸。可以区分出两组主要的三核苷酸：1）那些大多与停顿相关的；2）那些通常出现在无停顿区域的。第一组包括AUG、AUA、AUC、AAU、GUG、GUA、CGU、CGC、UUA、UUU；第二组包括AAA、CAA、CCC、UCC、CUA、CUG、CUC、GGG、ACU、GAG、GAA、GGA。已经建立了一个解释间歇性延伸的模型。它基于这样的假设，即每个核苷酸掺入到生长RNA链的3'-末端以及从其焦磷酸解的动力学常数取决于进入核苷酸的性质以及位于生长RNA 3'-末端的核苷酸残基的性质。提出了一个描述已知核苷酸序列的RNA上停顿分布的通用方程。
