脑组织中的差分脉冲伏安法。II. 大鼠纹状体中5-羟基吲哚乙酸的检测。

Differential pulse voltammetry in brain tissue. II. Detection of 5-hydroxyindoleacetic acid in the rat striatum.

作者信息

Cespuglio R, Faradji H, Riou F, Buda M, Gonon F, Pujol J F, Jouvet M

出版信息

Brain Res. 1981 Nov 2;223(2):299-311. doi: 10.1016/0006-8993(81)91143-4.

Abstract

Differential pulse voltammetry with electrochemically treated carbon fiber electrodes was used to study, in anesthetized rats, the alterations of the striatal electrochemical signal appearing at +300 mV (peak 3), following pharmacological manipulation of the animals. Decreases in peak 3 were obtained after injections of NSD 1015, Ro 4-4602 and Clorgyline. This, in conjunction with biochemical measurements, suggested that 5-hydroxyindoleacetic acid (5-HIAA) is, to a considerable extent, responsible for this electrochemical signal. Increases in peak 3 obtained after reserpine, L-tryptophan and D,L-5-HTP, as well as biochemical data reinforced this hypothesis, as did the detection of peak 3 in cerebrospinal fluid and its increase after probenecid injection. The occurrence of a feed-back mechanism is discussed following injection of D,L-5-HTP.

摘要

采用经电化学处理的碳纤维电极进行差分脉冲伏安法，在麻醉大鼠中研究动物经药理学处理后，纹状体在+300 mV时出现的电化学信号（峰3）的变化。注射NSD 1015、Ro 4-4602和氯吉兰后，峰3降低。这与生化测量结果相结合，表明5-羟吲哚乙酸（5-HIAA）在很大程度上是该电化学信号的原因。利血平、L-色氨酸和D,L-5-羟色氨酸注射后峰3升高，以及生化数据都强化了这一假设，脑脊液中峰3的检测及其在丙磺舒注射后的增加也强化了这一假设。注射D,L-5-羟色氨酸后讨论了反馈机制的发生。