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用促生长α球蛋白完全替代鸡胚脑细胞原代培养中的血清。

Complete replacement of serum in primary cultures of chick embryo brain cells by growth-promoting alpha-globulin.

作者信息

Baudysová M, Michl J

出版信息

Experientia. 1981;37(8):826-8. doi: 10.1007/BF01985662.

DOI:10.1007/BF01985662
PMID:6169539
Abstract

Growth-promoting alpha-globulin (GPAG), a specific serum protein complex which induces mitotic activity in continually replicating metazoan cells in vitro, was shown in this study to support growth of astrocytes and mesenchymal cells as well as process formation of nerve cells isolated from cerebral hemispheres of chick embryos.

摘要

促生长α球蛋白（GPAG）是一种特定的血清蛋白复合物，它能在体外诱导持续复制的后生动物细胞产生有丝分裂活性。本研究表明，它能支持星形胶质细胞和间充质细胞的生长，以及从鸡胚大脑半球分离出的神经细胞的突起形成。

相似文献

1
Complete replacement of serum in primary cultures of chick embryo brain cells by growth-promoting alpha-globulin.用促生长α球蛋白完全替代鸡胚脑细胞原代培养中的血清。
Experientia. 1981;37(8):826-8. doi: 10.1007/BF01985662.
2
Proliferation and differentiation of chondrocytes in cell culture.
Physiol Bohemoslov. 1983;32(4):346-51.
3
Differential expression in glial cells derived from chick embryo cerebral hemispheres at an advanced stage of development.发育晚期鸡胚大脑半球来源的神经胶质细胞中的差异表达。
J Neurosci Res. 1997 Feb 1;47(3):322-31.
4
Astrocyte differentiation is enhanced in chick embryos treated with ethanol during early neuroembryogenesis.
Neurochem Res. 1995 Sep;20(9):985-9. doi: 10.1007/BF00995550.
5
Histochemical study of dissociated nerve cells from embryonic chick cerebral hemispheres in flask cultures.鸡胚大脑半球解离神经细胞在培养瓶培养中的组织化学研究。
Experientia. 1973 Jun 15;29(6):699-701. doi: 10.1007/BF01944784.
6
A low-molecular weight chick brain-derived growth factor is mitogenic for cultured astroglia from the chick embryo.
Brain Res. 1988 Mar 1;467(1):97-104. doi: 10.1016/0165-3806(88)90070-3.
7
The effect of growth-promoting alpha-globulin (GPAG) on the incorporation of exogenous DNA into L-cells. I. Incorporation of isologous and heterologous 3H-DNA.促生长α球蛋白（GPAG）对外源DNA导入L细胞的影响。I. 同源和异源3H-DNA的导入
Neoplasma. 1978;25(1):37-46.
8
GABAergic neurons in cultures derived from three-, six- or eight-day-old chick embryo: a biochemical and immunocytochemical study.来自3日龄、6日龄或8日龄鸡胚培养物中的γ-氨基丁酸能神经元：一项生化与免疫细胞化学研究。
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9
Growth regulatory effect of the growth-promoting alpha-globulin in human diploid and heteroploid cells.
Physiol Bohemoslov. 1973;22(5):487-94.
10
Cholinergic neurons in cultures derived from three-, six- or eight-day-old chick embryo: a biochemical and immunocytochemical study.来自3日龄、6日龄或8日龄鸡胚的培养物中的胆碱能神经元：一项生化与免疫细胞化学研究。
Brain Res. 1988 May 1;468(1):37-46. doi: 10.1016/0165-3806(88)90005-3.

本文引用的文献

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Metabolism of cells in tissue culture in vitro. II. Long-term cultivation of cell strains and cells isolated directly from animals in a stationary culture.体外组织培养中细胞的代谢。II. 细胞株及直接从动物分离的细胞在静止培养中的长期培养。
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DIFFERENTIATION OF NEURONAL TYPES AND SYNAPSES IN MYELINATING CULTURES OF MOUSE CEREBELLUM.小鼠小脑有髓鞘培养物中神经元类型和突触的分化
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5
Metabolism of cells in tissue culture in vitro. I. The influence of serum protein fractions on the growth of normal and neoplastic cells.体外组织培养中细胞的代谢。I. 血清蛋白组分对正常细胞和肿瘤细胞生长的影响。
Exp Cell Res. 1961 Mar;23:324-34. doi: 10.1016/0014-4827(61)90042-8.
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Reconstituted rattail collagen used as substrate for tissue cultures on coverslips in Maximow slides and roller tubes.复溶鼠尾胶原蛋白用作在马克西莫夫载玻片和滚瓶中的盖玻片上进行组织培养的底物。
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8
Cultivation and growth of dissociated neurons from chick embryo cerebral cortex in the presence of different substrates.在不同底物存在的情况下，对鸡胚大脑皮层解离神经元的培养和生长。
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The behaviour of dissociated chick embryo brain cells in long-term cultures in presence and absence of brain extracts.
Differentiation. 1974 Apr;2(2):99-106. doi: 10.1111/j.1432-0436.1974.tb00341.x.
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Dissociation, fractionation, and culture of embryonic brain cells.胚胎脑细胞的解离、分级分离和培养。
Brain Res. 1969 Jan;12(1):180-99. doi: 10.1016/0006-8993(69)90065-1.