Whitington P M, Seifert E
J Comp Neurol. 1981 Aug 1;200(2):203-12. doi: 10.1002/cne.902000203.
The population of motorneurons which send axons to the metathoracic leg of the locust embryo has been stained by a cobalt backfilling technique. Embryogenesis lasts 11 days and an adultlike distribution of motorneuron cell bodies is seen as early as day 6: Several identified adult motorneurons can be recognized in the embryo. The total number of filled cells, the overall distribution of stained cell bodies, and the relative positions of identified neuron cell bodies do not change between 6 days and hatching. No motorneurons are stained which are clearly not leg motorneurons, and an adult pattern of axon branching at the level of peripheral nerve roots is already present at day 6. These observations provide information about the timing of motorneuron production and motor axon growth into the embryonic limb. The development of central dendritic arborizations can also be followed. The results are discussed with respect to mechanisms for neuron determination and differentiation.
通过钴回灌技术对向蝗虫胚胎后胸腿发送轴突的运动神经元群体进行了染色。胚胎发育持续11天，早在第6天就可以看到运动神经元细胞体呈现出类似成虫的分布：在胚胎中可以识别出几个已确定的成虫运动神经元。在第6天到孵化之间，填充细胞的总数、染色细胞体的整体分布以及已识别神经元细胞体的相对位置都没有变化。没有明显不是腿部运动神经元的运动神经元被染色，并且在第6天就已经出现了外周神经根水平的轴突分支的成虫模式。这些观察结果提供了有关运动神经元产生时间和运动轴突向胚胎肢体生长的信息。也可以追踪中央树突分支的发育。就神经元确定和分化的机制对结果进行了讨论。
