Murakami T, Mastaglia F L, Mann D M, Bradley W G
Muscle Nerve. 1981 Sep-Oct;4(5):407-12. doi: 10.1002/mus.880040509.
RNA metabolism was studied in anterior horn neurons in wobbler mice. In the wobbler mice, RNA content was reduced in histologically normal neurons but not consistently reduced in vacuolated neurons, as compared with normal littermates. Nuclear and nucleolar incorporation of [5--3H]uridine was reduced both in vacuolated and in histologically normal neurons of wobbler mice, while cytoplasmic counts were higher in histologically normal neurons of wobbler mice than in vacuolated neurons or the neurons of normal littermates. These findings point to an abnormality of RNA synthesis which precedes the earliest morphological changes seen in nerve cells with the light microscope. Nuclear [5--3H]uridine uptake was reduced both in small and in large neurons, a finding suggesting that the metabolic abnormality is probably not confined to alpha-motoneurons.
在摇摆小鼠的前角神经元中研究了RNA代谢。与正常同窝小鼠相比，在摇摆小鼠中，组织学上正常的神经元中的RNA含量降低，但空泡化神经元中的RNA含量并未持续降低。[5-³H]尿苷在摇摆小鼠的空泡化神经元和组织学上正常的神经元中的核内和核仁掺入均减少，而摇摆小鼠组织学上正常的神经元中的细胞质计数高于空泡化神经元或正常同窝小鼠的神经元。这些发现表明RNA合成异常先于光镜下神经细胞最早出现的形态学变化。小神经元和大神经元中的核[5-³H]尿苷摄取均减少，这一发现表明代谢异常可能不限于α运动神经元。
