Mourelle M, Rojkind M, Rubalcava B
Toxicology. 1981;21(3):213-22. doi: 10.1016/0300-483x(81)90157-8.
The adenylate cyclase system and the number and affinities of receptors for insulin and glucagon were studied in rats treated with CCl4 and in rats that received colchicine in addition to CCl4. Liver glycogen, cAMP and total collagen content were also measured in those animals. Rats received only mineral oil or only colchicine were used as controls. In this latter group, all parameters measured were normal. Differences in liver collagen content between groups treated with CCl4 and CCl4 + colchicine were not statistically significant. Basal adenylate cyclase activity was 2-fold increased in the CCl4 group. In the animals receiving CCl4 and colchicine, adenylate cyclase activity was normal. Adenylate cyclase activity stimulated by fluoride or by glucagon was also increased in the CCl4 group. However, this increase was due to to the enhanced basal activity. The number of receptors and the affinities of the receptors of glucagon and insulin were normal in all groups. cAMP levels were found increased in the CCl4 treated animals and this was accompanied by a 90% reduction in liver glycogen. In the group treated with CCl4 + colchicine, cAMP was normal and liver glycogen was only reduced 25%. These results suggest that part of the clinical and biochemical improvements observed in the colchicine treated animals were due in part to a reversal of the alterations of the adenylate cyclase system induced by CCl4.
在接受四氯化碳处理的大鼠以及除四氯化碳外还接受秋水仙碱处理的大鼠中，研究了腺苷酸环化酶系统以及胰岛素和胰高血糖素受体的数量与亲和力。还测量了这些动物的肝糖原、环磷酸腺苷（cAMP）和总胶原蛋白含量。仅接受矿物油或仅接受秋水仙碱处理的大鼠用作对照。在后者组中，所测量的所有参数均正常。四氯化碳处理组与四氯化碳加秋水仙碱处理组之间的肝胶原蛋白含量差异无统计学意义。四氯化碳组的基础腺苷酸环化酶活性增加了2倍。在接受四氯化碳和秋水仙碱的动物中，腺苷酸环化酶活性正常。四氯化碳组中由氟化物或胰高血糖素刺激的腺苷酸环化酶活性也增加。然而，这种增加是由于基础活性增强所致。所有组中胰高血糖素和胰岛素受体的数量及亲和力均正常。在接受四氯化碳处理的动物中发现cAMP水平升高，同时肝糖原减少了90%。在接受四氯化碳加秋水仙碱处理的组中，cAMP正常，肝糖原仅减少25%。这些结果表明，在接受秋水仙碱处理的动物中观察到的部分临床和生化改善部分归因于四氯化碳诱导的腺苷酸环化酶系统改变的逆转。
