可溶性免疫复合物中抗原鉴定的方法学探讨；一项模型研究。

Methodological approaches to antigen identification in soluble immune complexes; A model study.

作者信息

Husby S, Svehag S E, Nielsen H, Høiby N, Schiøtz P O

出版信息

Acta Pathol Microbiol Scand C. 1981 Apr;89(2):155-60. doi: 10.1111/j.1699-0463.1981.tb02679.x.

DOI:10.1111/j.1699-0463.1981.tb02679.x

Abstract

The usefulness of different techniques for identification of immune complex-(IC) bound antigen(s) was investigated in two Ab-Ag model systems. The following approaches were used: 1) determination of IC-activity before and after removal of IC by adsorption to antibody-specific solid phase systems. 2) analysis of PEG-insoluble IC by reference line-rocket immunoelectrophoresis and 3) reprecipitation of PEG-insoluble IC in the presence of 125I-labelled antibody to suspected antigens and subsequent analysis of the distribution of the radioactivity. This latter approach, the use of a double PEG precipitation immunoradiometric assay, was found most useful and simple for identification of antigen(s) contained in soluble IC. The technique is most sensitive in demonstrating IC-bound antigen(s) when the IC are formed in slight to moderate antigen excess.

摘要

在两个抗体-抗原模型系统中研究了不同技术对鉴定免疫复合物（IC）结合抗原的有用性。采用了以下方法：1）通过吸附到抗体特异性固相系统去除IC前后测定IC活性。2）通过参考线-火箭免疫电泳分析聚乙二醇（PEG）不溶性IC，以及3）在存在针对可疑抗原的125I标记抗体的情况下对PEG不溶性IC进行再沉淀，随后分析放射性分布。发现后一种方法，即使用双PEG沉淀免疫放射测定法，对于鉴定可溶性IC中所含抗原最为有用且简单。当IC在轻度至中度抗原过量情况下形成时，该技术在证明IC结合抗原方面最为敏感。