在胶固素结合试验中对可溶性免疫复合物进行抗原特异性检测。
Antigen-specific detection of soluble immune complexes in conglutinin-binding assays.
作者信息
D'Amelio R, Brighouse G, Barnet M, Lambert P H
出版信息
Clin Exp Immunol. 1981 Aug;45(2):283-9.
A modification of the conglutinin-binding assay has been used for antigen identification in circulating immune complexes (IC). The complexed antigen was identified, in conglutinin-bound complexes, by antibodies or antibody fragments, specifically directed against the antigen. These antibody preparations were either radiolabelled or detected with 125I-protein A. This system was found efficient using in vitro-formed bovine serum albumin--anti-bovine serum albumin and tetanus toxoid--anti-tetanus toxoid soluble immune complexes at equivalence or at slight antigen or antibody excess. In addition, with 125I-IgG--anti-HBs and with 125I-F(ab')2--anti-HBs, we examined 44 sera from 41 patients with viral type B hepatitis and the presence of HBs was observed in 18 (40.9%) IC containing tested samples. Therefore, this method appears applicable to clinical investigation.
已采用改良的胶固素结合试验来鉴定循环免疫复合物（IC）中的抗原。在胶固素结合的复合物中，通过特异性针对该抗原的抗体或抗体片段来鉴定复合抗原。这些抗体制剂要么用放射性标记，要么用¹²⁵I-蛋白A进行检测。在等当量或抗原或抗体略有过量的情况下，使用体外形成的牛血清白蛋白-抗牛血清白蛋白和破伤风类毒素-抗破伤风类毒素可溶性免疫复合物时，发现该系统是有效的。此外，用¹²⁵I-IgG-抗-HBs和¹²⁵I-F(ab')₂-抗-HBs，我们检测了41例B型病毒性肝炎患者的44份血清，在18份（40.9%）含检测样本的IC中观察到了HBs的存在。因此，该方法似乎适用于临床研究。
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