Heisler S, Chauvelot L, Desjardins D, Noel C, Lambert H, Desy-Audet L
Can J Physiol Pharmacol. 1981 Sep;59(9):994-1001. doi: 10.1139/y81-151.
Many calcium-mediated effects in mammalian cells may be activated by calcium-calmodulin stimulated enzymes. These effects are inhibited by various antidepressant drugs which bind to and inactivate calmodulin. In the current study, calmodulin was identified by affinity chromatography and gel electrophoresis in the cytoplasm of dispersed rat pancreatic acinar cells. Its role in enzyme secretion was assessed by evaluating the effects of various antidepressants drugs on the enzyme secretory process. Chlorpromazine, trifluoperazine, thioridazine, chlorprothixene and amitriptyline inhibited amylase secretion stimulated by carbachol, A-23187, and cholecystokinin-pancreozymin but not that elicited by dibutyryl cyclic AMP secretin or vasoactive intestinal peptide (VIP). Haloperidol, sulpiride, phenobarbital, and ethanol were without effect on secretagogue-stimulated enzyme release. Only those agents which blocked secretion also inhibited 45Ca release stimulated by carbachol from isotope preloaded cells. The data suggest that calmodulin may have a functional role in pancreatic enzyme secretion.
哺乳动物细胞中许多钙介导的效应可能由钙调蛋白刺激的酶激活。这些效应被各种与钙调蛋白结合并使其失活的抗抑郁药物所抑制。在当前的研究中，通过亲和层析和凝胶电泳在分散的大鼠胰腺腺泡细胞的细胞质中鉴定出了钙调蛋白。通过评估各种抗抑郁药物对酶分泌过程的影响来评估其在酶分泌中的作用。氯丙嗪、三氟拉嗪、硫利达嗪、氯普噻吨和阿米替林抑制了由卡巴胆碱、A-23187和胆囊收缩素-促胰酶素刺激的淀粉酶分泌，但不抑制由二丁酰环磷腺苷、促胰液素或血管活性肠肽（VIP）引起的淀粉酶分泌。氟哌啶醇、舒必利、苯巴比妥和乙醇对促分泌剂刺激的酶释放没有影响。只有那些阻断分泌的药物也抑制了由卡巴胆碱刺激的来自同位素预加载细胞的45Ca释放。数据表明钙调蛋白可能在胰腺酶分泌中具有功能性作用。
