Chevalier S, Bleau G, Roberts K D, Chapdelaine A
Mol Cell Endocrinol. 1981 Nov;24(2):195-208. doi: 10.1016/0303-7207(81)90059-9.
When cultured in monolayers, non-secretory epithelial cells from canine prostates actively synthesize DNA, RNA and proteins; subsequently, mitotic figures and an increase in cell number are observed. During this culture period, cell size and acid phosphatase activity remain constant. As the culture proceeds, these cells mature into secretory cells as evidenced by a gradual shift in their density in Percoll gradients to the density of secretory cells and by an increase in the cellular content of acid phosphatase. During maturation, the size of the non-secretory cells increases and their morphology changes and becomes similar to that of the secretory cells. When a homogeneous population of secretory cells is cultured, DNA synthesis is minimal and few mitotic figures may be observed while cell number and cell density remain constant. Early in the culture period, their size increases and by 2 weeks their acid phosphatase activity is 2--3-fold higher than that of the non-secretory cells. Thus, upon culture, the non-secretory epithelial cells enter and proceed through the cell cycle with evidence of DNA synthesis and mitosis. Those cells leaving the cycle undergo maturation into secretory cells which further differentiate with the concomitant appearance of acid phosphatase activity. This model will be useful to study prostatic hyperplasia and hypertrophy and the control mechanisms involved in these phenomena.
当在单层培养时，犬前列腺的非分泌性上皮细胞会积极合成DNA、RNA和蛋白质；随后，可观察到有丝分裂图像以及细胞数量增加。在这个培养期间，细胞大小和酸性磷酸酶活性保持恒定。随着培养的进行，这些细胞成熟为分泌性细胞，这表现为它们在Percoll梯度中的密度逐渐向分泌性细胞的密度转变，以及酸性磷酸酶的细胞含量增加。在成熟过程中，非分泌性细胞的大小增加，其形态发生变化并变得与分泌性细胞相似。当培养一群同质的分泌性细胞时，DNA合成极少，可能观察到的有丝分裂图像也很少，而细胞数量和细胞密度保持恒定。在培养期早期，它们的大小增加，到2周时其酸性磷酸酶活性比非分泌性细胞高2至3倍。因此，在培养时，非分泌性上皮细胞进入并经历细胞周期，有DNA合成和有丝分裂的证据。那些离开细胞周期的细胞成熟为分泌性细胞，这些分泌性细胞会随着酸性磷酸酶活性的出现而进一步分化。这个模型将有助于研究前列腺增生和肥大以及这些现象中涉及的控制机制。
