还原和非还原条件下人白细胞干扰素的大小特征

Size characteristics of human leucocyte interferon under reducing and non-reducing conditions.

作者信息

Braude I A, Lin L S, Stewart W E

出版信息

Biochem J. 1981 Mar 1;193(3):947-51. doi: 10.1042/bj1930947.

DOI:10.1042/bj1930947

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1162689/

Abstract

Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis was used to characterize human leucocyte interferon (HuIFN-alpha) under reducing and non-reducing conditions. Under non-reducing conditions HuIFN-alpha possesses two size forms, but under reducing conditions (r-HuIFN-alpha) only one is observed. The apparent molecular weight of this one form varies with the concentration of 2-mercaptoethanol used. When r-HuIFN-alpha is permitted to reoxidize the bimodal configuration of HuIFN-alpha is restored. The size heterogeneity of native HuIFN-alpha can be eliminated by mild treatment with NaIO4 [HuIFN-alpha/IO4; Stewart II, Lin, Wiranowska-Stewart & Cantell (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 4200-4204]. The size of the HuIFN-alpha/IO4 increases after treatment with 2-mercaptoethanol (r-HuIFN-alpha/IO4) and the apparent molecular weight of this component also varies with the concentration of 2-mercaptoethanol used. In the case of r-HuIFN-alpha the single peak observed apparently originates from both the higher- and lower-molecular-weight components.

摘要

采用十二烷基硫酸钠/聚丙烯酰胺凝胶电泳法在还原和非还原条件下对人白细胞干扰素（HuIFN-α）进行表征。在非还原条件下，HuIFN-α具有两种大小形式，但在还原条件下（r-HuIFN-α）仅观察到一种。这种单一形式的表观分子量随所用2-巯基乙醇的浓度而变化。当r-HuIFN-α重新氧化时，HuIFN-α的双峰构型得以恢复。天然HuIFN-α的大小异质性可以通过用NaIO4进行温和处理来消除[HuIFN-α/IO4；Stewart II、Lin、Wiranowska-Stewart和Cantell（1977年）美国国家科学院院刊74，4200 - 4204]。用2-巯基乙醇处理后（r-HuIFN-α/IO4），HuIFN-α/IO4的大小增加，并且该组分的表观分子量也随所用2-巯基乙醇的浓度而变化。就r-HuIFN-α而言，观察到的单峰显然源自高分子量和低分子量组分。

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