Stewart II W E, De Somer P, Edy V G, Paucker K, Berg K, Ogburn C A
J Gen Virol. 1975 Mar;26(3):327-31. doi: 10.1099/0022-1317-26-3-327.
Human fibroblast interferon preparations were completely stabilized to 100 degrees C by sodium dodecyl sulphate (SDS) in the presence of mercaptoethanol, but only a minor fraction of their activities were stabilized by SDS without mercaptoethanol. On the contarary, human leukocyte interferon preparations were completely stabilized to 100 degrees C by SDS in the absence of mercaptoethanol, but only a minor fraction of their activities were stabilized by SDS in the presence of mercaptoethanol. Furthermore, human fibroblast interferon preparations whose activities had been destroyed by boiling at 100 degrees C were completely reactivated by SDS under reducing conditions, but only a minor part of their activities were restored by SDS in the absence of reduction. On the contrary, human leukocyte interferon preparations whose activities had been destroyed by boiling at 100 degrees C were completely reactivated by SDS in the absence of reduction, but only a minor part of their activities were restored by SDS under reducing conditions. These data suggest that there are distinct molecular species of human interferons.
在巯基乙醇存在的情况下，十二烷基硫酸钠（SDS）可使人类成纤维细胞干扰素制剂在100℃下完全稳定，但在没有巯基乙醇时，只有一小部分活性可被SDS稳定。相反，在没有巯基乙醇的情况下，SDS可使人类白细胞干扰素制剂在100℃下完全稳定，但在有巯基乙醇时，只有一小部分活性可被SDS稳定。此外，在100℃煮沸已破坏活性的人类成纤维细胞干扰素制剂，在还原条件下可被SDS完全重新激活，但在没有还原的情况下，只有一小部分活性可被SDS恢复。相反，在100℃煮沸已破坏活性的人类白细胞干扰素制剂，在没有还原的情况下可被SDS完全重新激活，但在还原条件下，只有一小部分活性可被SDS恢复。这些数据表明，人类干扰素存在不同的分子种类。
