Yonezawa H, Kaneda M, Tominaga N, Higashi S, Izumiya N
J Biochem. 1981 Oct;90(4):1087-91. doi: 10.1093/oxfordjournals.jbchem.a133560.
Gramicidin S (GS) containing 14C-labeled proline was synthesized by a solid-phase method, and the labeled GS dihydrochloride was obtained as crystals. The labeled GS exhibited same antibacterial activity as natural GS. Strains sensitive to GS (B. subtilis and S. aureus) and an insensitive strain (E. coli) were treated with the labeled GS, and the amount of the labeled GS adsorbed on the cells was measured. GS was adsorbed rapidly on the cells of the sensitive strains; the amount adsorbed increased linearly with GS concentration up to 1-1.5 microgram/ml, and at a lower rate at above 1.5 microgram/ml. GS molecules covered most of the cell surface at the minimum inhibitory concentration of 1.5 microgram/ml; the number of molecules adsorbed per cell was 1.3-1.4 x 10(6). No GS was adsorbed by the insensitive strain.
采用固相法合成了含14C标记脯氨酸的短杆菌肽S（GS），并得到了标记的盐酸GS晶体。标记的GS表现出与天然GS相同的抗菌活性。用标记的GS处理对GS敏感的菌株（枯草芽孢杆菌和金黄色葡萄球菌）和不敏感的菌株（大肠杆菌），并测定吸附在细胞上的标记GS的量。GS能迅速吸附在敏感菌株的细胞上；吸附量随GS浓度增加呈线性增加，直至1-1.5微克/毫升，在1.5微克/毫升以上时增加速率较低。在最低抑菌浓度1.5微克/毫升时，GS分子覆盖了大部分细胞表面；每个细胞吸附的分子数为1.3-1.4×10(6)。不敏感菌株不吸附GS。
