Davies P F, Kuczera L
J Histochem Cytochem. 1981 Dec;29(12):1437-41. doi: 10.1177/29.12.6172467.
Ruthenium red staining of plasma membrane glycoproteins of confluent cultured arterial endothelial cells revealed that the limiting membrane of many apparently discrete cytoplasmic vesicles was continuous with the plasmalemma. Surface invaginations accessible to ruthenium red appeared as vesicles when sectioned out of the plane of attachment to the cell surface, Morphometric analysis of ruthenium red-positive (RR+) and ruthenium red-negative vesicles (RR-) indicated that 47.2% of the total apparent vesicle population was RR+ and that those infoldings accounted for 19.6 +/- 1.4% of the cell surface in transverse sections. Whereas 14.9% of the true vesicles (ruthenium red-negative) were coated vesicles, only 1.1% of RR+ "vesicles" were coated pits. These studies show that although many deep infoldings of the cell surface may be misinterpreted as vesicles, almost all are uncoated. The existence of discrete coated vesicles (independent of coated pits) in vascular endothelium in vitro is readily apparent.
对汇合培养的动脉内皮细胞质膜糖蛋白进行钌红染色显示，许多明显离散的细胞质囊泡的界膜与质膜连续。从与细胞表面附着平面切出时，钌红可进入的表面内陷呈现为囊泡。对钌红阳性（RR+）和钌红阴性囊泡（RR-）的形态计量分析表明，总的明显囊泡群体中47.2%为RR+，在横切面上，那些内褶占细胞表面的19.6±1.4%。虽然14.9%的真正囊泡（钌红阴性）是有被囊泡，但只有1.1%的RR+“囊泡”是有被小窝。这些研究表明，尽管细胞表面的许多深内褶可能被误解为囊泡，但几乎所有都是无被的。体外血管内皮中离散有被囊泡（独立于有被小窝）的存在很明显。
