Rosenberg J S, Gilman S C, Feldman J D
J Immunol. 1982 Feb;128(2):656-60.
Anti-mu, anti-gamma, and anti-delta antibodies induce proliferation of splenic B lymphocytes from young Lewis rats, measured by 3H-TdR uptake. In contrast, splenic B cells of aged Lewis rats respond poorly or not at all to these reagents. T lymphocytes or interleukin 2 (IL-2) of young or aged rats augment the uptake of 3H-TdR in cultures of "young" B cells responding to anti-Ig reagents or LPS and DxS, but have no significant effect on the responses of "old" B cells. Analysis of spleen cells of young and aged rats in a fluorescence-activated cell sorter indicates the density of mu, gamma, and delta isotypes is reduced in "old" B cells, and that B cells of aged rats are significantly larger than those of young rats. These results delineate anatomic and structural changes in B lymphocytes of aged rats.
通过³H-TdR摄取量测定发现，抗μ、抗γ和抗δ抗体可诱导幼年Lewis大鼠脾B淋巴细胞增殖。相比之下，老年Lewis大鼠的脾B细胞对这些试剂反应不佳或根本无反应。幼年或老年大鼠的T淋巴细胞或白细胞介素2（IL-2）可增强“幼年”B细胞在对抗Ig试剂或LPS及DxS产生反应的培养物中³H-TdR的摄取，但对“老年”B细胞的反应无显著影响。在荧光激活细胞分选仪中对幼年和老年大鼠的脾细胞进行分析表明，“老年”B细胞中μ、γ和δ同种型的密度降低，且老年大鼠的B细胞明显大于幼年大鼠的B细胞。这些结果描绘了老年大鼠B淋巴细胞的解剖学和结构变化。
