Identification by affinity chromatography of wheat germ ribosomal proteins which interact with RNA.

Wheat germ ribosomal proteins that bind to poly(U), tRNA and poly(A)-containing mRNA for leghaemoglobin were identified by affinity chromatography. The ribosomal proteins that associate with polynucleotides immobilized on Sepharose or on cellulose were identified by polyacrylamide gel electrophoresis. Nine 40S and nine 60S ribosomal proteins were bound to poly(U)-Sepharose. tRNA-Sepharose 4B interacted with five proteins of 40S subunit and with seven proteins of 60S subunit. Six proteins of 60S subunit were bound to poly(A)-containing mRNA for leghaemoglobin. The ribosomal proteins from the small subparticle were not determined because of irreversible binding to the column.