Sakamoto C, Otsuki M, Ohki A, Yuu H, Maeda M, Yamasaki T, Baba S
Endocrinology. 1982 Feb;110(2):398-402. doi: 10.1210/endo-110-2-398.
The effect of pure natural porcine cholecystokinin (CCK) and synthetic caerulein on endocrine and exocrine pancreatic secretion was investigated in the isolated perfused rat pancreas in the presence of physiological concentrations of glucose. CCK (0.25 mU/ml) or caerulein (0.01 ng/ml) potentiated the insulin secretion induced by 5.6 mM glucose; a significant increase in pancreatic exocrine secretion was also observed at these doses of CCK or caerulein. Further increases in the concentration of CCK (0.25 to 1 mU/ml) or caerulein (0.01 to 1 ng/ml) resulted in dose-dependent increases in both insulin and pancreatic exocrine secretion. The effectiveness of CCK and caerulein as insulinotropic agents depended on the glucose concentration; they were more effective at higher concentrations of glucose. Thus, CCK or caerulein significantly and coincidentally stimulated both insulin secretion and pancreatic exocrine function if 5.6 mM or more glucose is present, whereas in previous studies using 2.8 mM glucose, stimulation of insulin secretion was elicited only with concentrations of the peptides supramaximal for an effect on pancreatic exocrine secretion. CCK may contribute to the entero-insular axis.
在生理浓度葡萄糖存在的情况下，利用离体灌注大鼠胰腺研究了纯天然猪胆囊收缩素（CCK）和合成蛙皮素对胰腺内分泌和外分泌功能的影响。CCK（0.25 mU/ml）或蛙皮素（0.01 ng/ml）增强了5.6 mM葡萄糖诱导的胰岛素分泌；在这些剂量的CCK或蛙皮素作用下，胰腺外分泌也显著增加。CCK浓度进一步升高（0.25至1 mU/ml）或蛙皮素浓度进一步升高（0.01至1 ng/ml）导致胰岛素和胰腺外分泌呈剂量依赖性增加。CCK和蛙皮素作为促胰岛素分泌剂的有效性取决于葡萄糖浓度；它们在较高葡萄糖浓度时更有效。因此，如果存在5.6 mM或更高浓度的葡萄糖，CCK或蛙皮素会显著且同时刺激胰岛素分泌和胰腺外分泌功能，而在先前使用2.8 mM葡萄糖的研究中，仅在对胰腺外分泌分泌产生作用的肽浓度超过最大剂量时才会引发胰岛素分泌的刺激。CCK可能参与肠-胰岛轴。
